The Gene Pulser Xcell™ is a modular electroporation system that includes a main unit, two accessory modules (the capacitance extender, or CE module, and . The Gene Pulser Xcell total system is the complete electroporation system for transfection of both eukaryotic and prokaryotic cells. The Gene Pulser Xcell total. We have one Year BIO-RAD Gene Pulser xCell Total Electroporation System for sale. Lab paid $ in Year but had to close.
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The catalog number, SKU number, or product number is printed on your product label. Considerations for troubleshooting are also discussed in the associated video. Exponential or square Voltage: In some experiments the largest number of transfected cells might be optimal, in other experiments the highest percentage transfection might be best. Detailed parameters can be found for each of these cell lines, with details for the preparation of electrocompetent cells in the Gene Pulser Xcell system instructions or at www.
Where can I find the catalog number, SKU number, or product number? Visit Bio-Rad Laboratories, Inc. All pictures shown above are of actual product s sold. Mix cuvette contents by pipetting up and down in cuvette.
If your answer is yes, xcll protocol should we use for the program? Interact with Bio-Rad Laboratories, Inc. Check flasks with a microscope to verify the condition of the cells, smack flask to detach cells, and then check flask again to make sure cells are all detached. Please select your country below to view the products, information, and services available in your location, including news, promotions, events, and more.
Gene Pulser: Analytical Instruments | eBay
If the problem persists, please call customer service at or send an email to. Here the puler of cells that are GFP positive in the passage 5 cells under each of the 12 electroporation conditions are shown. Also shop in Also shop in. Truncating the pulse from a capacitor after discharging it xecll the sample generates a square-wave pulse. July 31 st1 Reply Post a Reply. Please try reloading the page.
There is a good chance that you will achieve success with the conditions used here although they may not be the absolute best conditions for your particular MEFs. The lot number or control number one or the other, not both is printed on your product label. Number of bids and bid amounts may be slightly out of date. Gene Pulser Xcell to My Bench. pulset
Skip to content Biology. Manuals and Resources Note: Let cells recover for 24 hours. Item was pulled from a working environment comes with power cord onlyit have some scratches and marks but is fully working.
BIO-RAD Gene Pulser xCell Total Electroporation System Year 2012
pulsee In these situations, the Certificate of Analysis is available for download, but other product details, such as the product name, are not. Put plate in plate chamber and pulse. We take out all the guess work so you don’t have to.
We will get back to you as soon as possible. When using our online Certificate of Analysis search tool, keep the following in mind: Flow cytometry can provide quantitative information about the puler results under each of the different electroporation conditions.
For questions about using the LabWrench web site please call us. Outputs and other specifications: How do I apply for the education discount?
I hope that helps! The CE module is recommended for use with the Gene Pulser Xcell main unit for electroporation of most eukaryotic cells, including mammalian cells and plant protoplasts. Can I get a Certificate of Analysis even if my item is expired? Let the cells recover for 24 pusler. Moreover, how will the size of the cuvettes and volume of cell suspension influence the transfection efficiency? Gwne am not overjoyed with what’s happening, but I have to roll with it.
Viewing the cells under phase contrast allows visualization of both transfected and untransfected cells.