The purpose of the study was to validate the Epsilometer test (E-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms. ETEST is a well-established method for antimicrobial susceptibility testing in microbiology laboratories around the world. ETEST consists of a predefined. We describe the applicability of the E test (AB Biodisk Solna, Sweden), a new method for determining minimum inhibitory concentrations of antimicrobial agents.
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In addition, specific Etest products are available for the detection of specific resistance mechanisms [e.
E Test: a novel technique for antimicrobial susceptibility testing
Etest applications include many groups of fastidious organisms, fungi yeast and mould and mycobacteria as well as detecting various mechanisms of resistance and MIC testing of key antibiotics with critical specimens e. The E test approach may be well suited for the testing of certain fastidious bacteria or bacteria that is difficult to epzilometer.
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Learn how and when to remove these template messages. The E-test ICs for ampicillin, cefoxitin, and metronidazole against the Gram-negative capnophilic and microaerophilic rods and the black-pigmented anaerobic rods ICs for ampicillin, clindamycin, metronidazole, and tetracycline showed a high percentage of agreement with the agar dilution MICS.
National Committee for Clinical Laboratory Standards. Diagn Microbiol Inf Dis, Like the agar disk diffusion method, the E test is easy to execute. Additionally, the simultaneous testing of several isolates is possible usually 20 to 37 epsilometeg microbial contamination or heterogeneity is more readily detected than broth methods.
After 48 hours incubation a drop-shaped inhibition zone intersects the graded test strip at the inhibitory concentration IC of the antibiotic. Additionally, it is able to retain some of the favorable principles of agar dilution testing by simply producing quantitative MICs.
The purpose of the study was to validate the Epsilometer test E-test method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard.
However, the results of such measurements are not an absolute value because they are influenced sometimes markedly, by the test conditions used. Waging war on the deadliest superbug.
The deficiencies of the disk diffusion test are its non-quantitative interpretation and its inapplicability to many fastidious organisms and anaerobes. Antimicrobial susceptibility testing may be done by a variety of techniques.
E-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms.
E test strip is a non-porous plastic strip immobilized with predefined continuous and stable gradient of 15 antibiotic concentrations on one side and printed with an MIC scale on the other side of nylon strips. Now FDA k Cleared! Antibiogram, E test, susceptibility. A report of an international collaborative study. Other methods for susceptibility testing include: One or more E test strips for the antimicrobial agents to be tested are then placed on the inoculatated agar surface.
The authors are grateful to Dr.
How to cite this article. The most frequently used method in Brazil is disk diffusion 3, United States of America. Etest products for more than antimicrobial agents, including antibiotics, antifungal agents and antimycobacterial agents are available.
The Etest family of instruments is designed to simplify the daily use of Etest. It is technically simple and requires careful attention to de-tails, leading to reproducible results. After an overnight incubation, the interaction of the antimicrobial agent gradient and the test bacterial inoculum gives rise to elliptical inhibitory zones figure 1.
Etest and related instruments offer one of the most efficient methods for generating on-scale MIC values across 15 doubling dilutions for susceptibility testing of a wide range of drug-bug combinations, including fastidious organisms. In the study of Jorgensen et al, Streptococcus penumoniae and 50 Hemophilus influenzae that demonstrated various resistance mechanisms and levels of anti-microbial suscetibility were examined by E tests performed on agar media currently recommended by the NCCLS HTM and Muller-Hinton sheep blood agars, respectively.
The E-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. Measurement of the susceptibility of microorgan isms to antimicrobial agents is of great impor tance in the rational use of antimicrobial agents, in the evaluation of new drugs, and in epidemiological studies. Meningitis due to mixed infection with penicillin-resistant and penicillin-susceptible strains of Streptococcus pneumoniae.
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We describe the applicability of the E test AB Biodisk Solna, Swedena new method for determining minimum inhibitory concentrations of antimicrobial agents against bacteria. Dilution testing by agar method is also standardized method and a reliable susceptibility testing technique that may be used as a reference for evaluating the accuracy of other testing systems.
The E test is of particular interest to the clinical laboratory because it allows the determination of the susceptibility MIC of an organism to one or more selected antibiotics rather than to a predetermined panel of antimicrobial agents. J Clin Microbiol, It can also be used in a macromethod format to optimize resistance detection. Your email address will not be published. The use of microdilution trays prepared in house allows simultaneous testing of several antimicrobial agents against individual organisms and also provides a reliable standardized reference method for susceptibility testing.
It is particularly helpful in determining choice and dosage of antimicrobials in patients with sterile site infections i. Simplex C76, Nema C88, and Retro C80 are easy to use, reducing operator fatigue, saving time and improving the quality of results by increasing reproducibility. This report is based on the literature review and on our own experience using the E test for susceptibility testing of the Xanthomonas maltophilia, Streptococcus pneumoniae and Streptococcus viridans group against eight different drugs.
A total of 60 strains of key periodontal pathogens Prevotella intermedia, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Eikenella corrodens, Campylobacter rectus, and Capnocytophaga isolated from 22 sites of 12 patients were studied. Accessed October 24,