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(a) Principle of the reactions underlying DDRT-PCR analysis. (N) Any of the four nucleotides G, A, T, or C. V can be any nucleotide besides T. (B) Any nucleotide. Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to. Establishment of infection and disease implies modifications in the genetic programmes of the cell systems that are involved and the differential expression of.

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The number and extent of the genes affected is not totally known to others. These results confirm the common concept regarding the ability of microorganism to develop new strategies to resist more and more antibiotics, which invite the researchers to find new and effective antimicrobial agents. Extraction of the bioactive metabolites produced by SA isolate The extraction of the active metabolites produced by the endophytic isolate SA was done as follows: The extraction of the active metabolites produced by the endophytic isolate SA was done as follows: Antagonistic effect of endophytic isolate SA against S.

Differential display using Ea1 and Ea2 primers combined with OP1 reverse transcription primer. Europe PMC requires Javascript to function effectively. Using of ethanol and sodium hypochlorite at certain dilutions is recommended for complete removal of the plant surface bacteria Jan et al. In our study, four up-regulated and down-regulated genes were chosen for gel purification and sequencing. In-vivo expression technologies and differential display RT-PCR are providing new approaches to further examine a microbe’s response to experimental conditions which more closely resemble natural microbial associations and habitats.

Gene Ontology GO Terms.

Differential display

To our knowledge, drdt is the first study that illustrates the bacterial production of serine protease for antagonistic function against another genus of bacteria. In vitro antifungal activities of inhibitors of phospholipases from the fungal pathogen Cryptococcus neoformans. In the latter case, please turn on Javascript support in your web browser and reload this page.


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Components in the ethanol extract of inhibition zone slices GC—MS chromatogram analysis of the ethanol extract of inhibition zone parts s howed eight peaks that led to the identification of a number of compounds Fig.

Serine proteases from nematode and protozoan parasites: Support Center Support Center. Exploring these novel compounds and metabolites may lead to the discovery of new drugs for antibiotic resistant pathogenic bacteria like methicillin resistant Staphylococcus aureus MRSA.

The discovery of these affecting genes is related to the sequence of the primers used during the reverse transcription process.

About ten bacterial colonies with the same colony shape and characteristics were obtained. However, only one of the sequenced genes was matched with genes deposited in GenBank. Serine protease is also known to be produced by bacteria for purposes oth er than bacterial—bacterial antagonistic effect, which has been confirmed by this study.

Khan Z, Doty SL. The two bacterial strains the endophytic isolate SA and S. The cultivated endophytic bacterial isolates were transferred to new nutrient agar NA plates for purification.

Differential display – Wikipedia

This study focuses on the isolation of endophytic bacteria which are able to cease and inhibit the growth and spreading of methicillin resistant S. CitePeer Related Articles http: Plant surface sterilization is ddrt to avoid the co-contamination with superficial bacteria. Articles from 3 Biotech are provided here courtesy of Springer. Moustafa1, 2 Tarek H. Antagonistic effect of SA isolate against S.

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The antagonistic activity of the endophytic bacterial isolate was compared with ten different known bacterial antibiotics against S. They play an important role in the plant health preservation in addition to their ability to supplement their host plants with inorganic nutrients via nitrogen fixation and iron solubilization processes Porras-Soriano et al.

Acknowledgments We would like to thank King Khalid University, Faculty of Science, Biology Department for supporting this work with all the required chemicals and equipments. This factor has a broad spectrum of activity against Gram-positive and specifically against Staphylococcus aureus MRSA. National Center for Biotechnology InformationU. Either your web browser doesn’t support Javascript or it is currently turned off.


Both of the pathogenic strain S. In vitro antifungal activity of Argemone ochroleuca Sweet latex against some pathogenic fungi. Both of sterile filter paper discs were loaded with the isolate SA and ten different antibiotic discs. Find all citations in this journal default. Multiple sequence alignment, molecular ddrt and phylogenetic tree were performed using MEGA 5 software Fig. Materials and methods Plant materials Salvadora persica L.

Phytochemical and pharmacological screening of Sterculiaceae species and isolation of antibacterial compounds. Identification of the producer strain was performed using the partial nucleotide sequence of 16S rRNA gene, which indicated that this strain is identical to B.

The grown separate pure colonies of bacterial isolate were tested for their antagonistic activity against Klebsiella pneumoniaeEscherichia ddrtt and methicillin resistant S.

The production of antibiotics and hydrolytic enzymes is a feature of many endophytic bacilli, pvr Bacillus cereus Pleban et al. To investigate the upregulated or downregulated genes during the antagonistic process, both of the pathogenic and the antagonistic bacteria were grown either separately on two different nutrient agar plates or close to each other on the same plate.

The obtained ddt were compared with the sequences deposited in GenBank data base http: The antagonistic factor was isolated from the bacterial culture medium and purified by thin layer chromatography technique, then analyzed by GC-MS analysis.

Miswak stem parts were surface sterilized followed by the isolation of inner bacterial isolates on NA plates. Isolation, gene cloning and expression profile of a pathogen recognition protein: Bacillus subtilis BS edrt an antagonist of potato blackleg and soft rot bacteria.